International Activity

Evolutionary theory for constrained and directional diversities

Shigeru Kuratani (RIKEN, Chief Scientist)

Research Project Number:17H06384  Researcher Number: 00178089

Evolutionary theory for constrained and directional diversities

Shigeru Kuratani (RIKEN, Chief Scientist)

Research Project Number:17H06384  Researcher
Number: 00178089

【Purpose of the Research Project】

Living organisms do not evolve perfectly in random directions, but we recognize unevenness and directionalities in phenotypic variations and evolutionary changes.
Thus far, however, these directionality or evolutionary constraints have never been dealt with scientifically. In this project, we aim at detecting them at various hierarchical levels, to understand the relationships or correlations between phenotypic changes observed in a short time scale (like adaptive responses) and those observed in a longer scale as evolution, to look for mechanistic and causal logics linking between the two, and finally to establish a theoretical framework to deal with the patterns of evolution, involving classical natural selection and neutral theories.

【Content of the Research Project】

1.To quantify the phenotypic response towards environmental and developmental perturbations, and to speculate the mode of correlation between the responsive gene expression levels and the responding phenotypic variations based on the fluctuation - response theory already developed in the field of physics. Thus, we will describe and measure the correlation between responses of gene regulation and phenotypes, and fluctuating embryonic morphological patterns and resultant phenotypic variations. Variations in epigenetic regulation will also be the target of the analyses.
2. To speculate the existence of constraints in the process of phenotypic generation during evolution, by means of comparison of phenotypes among various species, and to analyse, behind the fluctuating phenotypes, how the responsible genes' expressions and developmental patterns can fluctuate. Using simple models, we aim at performing experimental evolutionary analyses (E. coli), to identify and quantify genetic factors (structure of gene expression networks) behind the constrained phenotypes.
3. Based on the results obtained in 1 and 2, and by integrating the simulation analyses using simple models as well, we aim at exploiting methodologies to analyse the relationship between phenotypic variations and evolutionary changes, as the bases for applying to multiple systems at different hierarchical levels including complicated body plans and host-parasite symbiosis.
Eventually, we will try to establish a new theoretical framework to deal with evolutionary directionality and constraints.

【Expected Research Achievements and Scientific Significance】

One major aspect of this project is the fact that it involves concepts of statistic physics to deal with so far untouchable questions in the evolutionary biology, namely, the causal and mechanistic nature of evolutionary directionalities and constraints.
We also aim at establishing an integrative theory that can deal with evolutionary phenomena at various, multiple hierarchical levels like, from molecules and cell to the complex anatomical traits or ecological level evolution. The hint is already given from the field of physics and theoretical biology (fluctuation-response evolutionary theory; Kaneko and Furusawa, 2006). Not a while ago, even the quantum physics could never imagine that the field would ever be related by itself directly to the evolution of the entire universe, but now it does.
Integration of the theory and observation has connected the two different worlds, which used to look so distantly related from each other.
In the present project also, it is aimed at jumping over different hierarchical levels of phenomena, to show how a simple and integrative rule governs the entire world of organismal evolution and perplexing diversity surrounding us.

【Key Words】

Evolutionary biology, Evo-Devo, Evolutionary morphology, Experimental evolutionary biology, Ecology, Physics, Theoretical biology.

【Term of Project】


【Related Meeting】

AsiaEvo (We have co-organized the 1st AsiaEvo in 2018@Shenzhen)

【CDE Online open seminar】

【Research groups】

Planned Research A01
Principal Investigator
Shigeru Kuratani (RIKEN)
Lab website
Co-Investigator  Juan Pascual Anaya (RIKEN), Tatsuya Hirasawa (RIKEN)
Planned Research A02
Principal Investigator
Kunihiko Kaneko (Univ. of Tokyo)
Lab website
Co-Investigator  Koichi Fujimoto (Osaka Univ.), Nobuto Takeuchi (Univ. of Tokyo)
Planned Research A03
Principal Investigator
Naoki Irie (Univ. of Tokyo)
Lab website
Research collaborator  Yui Uchida (Univ. of Tokyo PhD course student)

start-up member
Planned Research A04
Principal Investigator
Takema Fukatsu (AIST)
Lab website
Co-Investigator  Shuji Shigenobu (NIBB), Ryuichi Koga (AIST), Naruo Nikoh(The Open Univ. Japan), Takahiro Hosokawa (Kyushu Univ.)
Planned Research A05
Principal Investigator
Chikara Furusawa (RIKEN, Univ. of Tokyo)
Lab website
Co-Investigator  Yuichi Wakamoto (Univ. of Tokyo)
Planned Research A06
Principal Investigator
Mitsuyasu Hasebe (NIBB)
Lab website
Research collaborator  Kenji Fukushima (JSPS Postdoc), Gergo Palfalvi(SOKENDAI PhD course Student)
Planned Research A01
Principal Investigator
Shigeru Kuratani (RIKEN) 
 Lab website
Co-Investigator        Juan Pascual Anaya (RIKEN), Tatsuya Hirasawa (RIKEN)
Planned Research A02
Principal Investigator
Kunihiko Kaneko (Univ. of Tokyo)
 Lab website
Co-Investigator        Koichi Fujimoto (Osaka Univ.)
Research collaborator Nobuto Takeuchi (Univ. of Tokyo)
Planned Research A03
Principal Investigator
Naoki Irie (Univ. of Tokyo)
 Lab website
Research collaborator   Yui Uchida (Univ. of Tokyo PhD course student)
Planned Research A03
Principal Investigator
Naoki Irie (Univ. of Tokyo)
 Lab website
Research collaborator   Yui Uchida (Univ. of Tokyo PhD course student)
Planned Research A05
Principal Investigator
Chikara Furusawa (RIKEN, Univ. of Tokyo)
 Lab website
Co-Investigator        Yuichi Wakamoto (Univ. of Tokyo)
Planned Research A06
Principal Investigator
Mitsuyasu Hasebe (NIBB) 
 Lab website 
Research collaborator  Kenji Fukushima (JSPS Postdoc), Gergo Palfalvi(SOKENDAI PhD course Student)

【Published Dataset】

Whole embryonic RNAseq data of chordates

Brief Description: Early-to-late whole embryonic gene expression profiles obtained from 8 chordate species (B. floridae, C. intestinalis, D. rerio, G. gallus, M. musculus, P. sinensis japonicus, X. laevis, X. tropicalis).
Link to the dataset: Sequence Read Archive[DRA] DRA003460 , SRA database
Related Publication : Nature Ecology & Evolution,1, 1722-1730 (2017)

Hagfish Genome Project

Brief Description: We have sequenced the germline genome of the inshore hagfish Eptatretus burgeri in order to provide a reference genome of this species, which represents a group of vertebrates for which no genome has been provided so far. Together with the reference genome, we provide the genome sequence data and RNA-seq data of 8 somatic tissues aiming at studying putative somatic genome rearrangements.
Link to the dataset: BioProject, Genome Assembly
Related Publication :In preparation.

Vertebrate Hox temporal collinearity

Brief Description: Here we provide a developmental series of RNA-seq data from 3 different species, the hagfish Eptatretus burgeri, the lamprey Lethenteron camtschaticum, and the shark Scyliorhinus torazame, encompassing the two major groups vertebrates, cyclostomes and gnathostomes. Comprehensive analysis of the data allowed us to demonstrate that the Hox genes of the last common ancestor of vertebrates were expressed according to the classical temporal collinearity.
Link to the dataset: BioProject
Related Publication : Nature Ecology & Evolution,2, 859-866 (2018)

【News Letters】

 Vol.2 No.S3En
Repeated inversions within a pannier intron drive diversification of intraspecific colour patterns of ladybird beetles

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 Vol.2 No.S2En
Recurrent symbiont recruitment from fungal parasites in cicadas

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 Vol.2 No.S1En
Hagfish and lamprey Hox genes reveal conservation of temporal colinearity in vertebrates

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 Vol.1 No.S2En
Physcomitrella MADS-box genes regulate water supply and sperm movement for fertilization

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